To demonstrate the validity of Transcriptic’s Autoprotocol-implemented High Throughput Pipetting Quick Instruction.
Orange G electrophoresis dye (CHEM IMPEX) solutions of 33, 67 and 100 µg/ml were prepared in distilled water. Thirty microliters of dye were transferred using the High Throughput Pipetting Quick Instruction from 96-well source plates to 384-well analysis plates and underwent absorbance measurement (475 nm) as an assessment of pipetting accuracy.
Replicate transfers (n=96) performed using the High Throughput Pipetting Quick Instruction achieved minimal standard deviations for all 4 solutions (Figure 2). A linear relationship between absorbance and Orange G volume is observed with increasing dye concentration.
VALIDATION DATE: September 2015