To demonstrate the validity of Transcriptic’s Autoprotocol-implemented Plasmid Preparation Quick Instruction.
E. coli (Mix & Go Zymo5ɑ, Zymo) with pBabe-puro-encoded  ampicillin resistance were grown and then processed for DNA extraction and purification using the Plasmid Preparation Quick Instruction. As a comparison control, the same E. coli strain lacking plasmid-encoded resistance was similarly grown and processed. DNA yields were determined using a NanoDrop Lite Spectrophotometer (Thermo Scientific).
The Plasmid Preparation Quick Instruction was employed to successfully inoculate and propagate bacterial stocks with successful, high-yield (Figure 1) DNA extraction.
 Morgenstern JP, Land H. Advanced mammalian gene transfer: high titer retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 1990;12:3587-96
VALIDATION DATE: September 2015