This protocol automates PCR on the Transcriptic platform.
This workflow takes an empty CRISPR vector of your choice along with oligos based on your guide sequence and delivers final assemblies - ready for you to transform - 96 at a time.
This protocol automates colorimetric protein detection from liver tissue extracts or plasma samples using enzyme-linked immunosorbent assay (ELISA) technology on the Transcriptic platform.
This protocol automates ExoSap-IT treatment for PCR purification on the Transcriptic platform.
This protocol automates Genotyping on the Transcriptic platform.
This method uses a phage-derived uracil-containing ssDNA template and mutagenic oligonucleotides to confer targeted mutations to a plasmid of interest.
The Oligosynthesis Quick Instruction generates high quality (mass spectroscopy and capillary electrophoresis-verified) oligonucleotides.
The Picogreen Protocol successfully generated a standard curve using standards of known DNA concentration (0-50 ng/µl). Plotting of log10[DNA concentration] vs. fluorescence generated a linear trend.
This protocol automates High-Throughput Pipetting treatment for PCR purification on the Transcriptic platform.
The Plasmid Preparation Quick Instruction can be used to inoculate and propagate bacterial stocks with successful, high-yield DNA extraction.
Assessment of plate reading measurements employing multiple wavelengths between 475 - 535 nm was achieved using two independent experiments, performed in the same measurement plate (96-well, flat-bottom, optically-clear).
This protocol automates RTqPCR on the Transcriptic platform.
This protocol automates Restriction Digestion on the Transcriptic platform.
This protocol automates RNA isolation from bacterial cultures and mouse liver tissue samples using magnetic bead-based technology on the Transcriptic platform.
This protocol automates Sequencing on the Transcriptic platform.
Transform, Spread, Pick
This protocol takes up to 32 DNA aliquots of your choice and Zymo Mix & Go  (Zymo Research Corporation) chemically competent cells (Zymo 5α, Zymo 10B, or JM109) to generate individually picked colonies from streaked bacteria.
This protocol automates the QuikChange Lightning kit, which provides a highly efficient non-PCR method for reliable site-directed mutagenesis.