TECHNICAL NOTES

ELISA

This protocol automates colorimetric protein detection from liver tissue extracts or plasma samples using enzyme-linked immunosorbent assay (ELISA) technology on the Transcriptic platform.

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MSD-Assay

This protocol automates colorimetric protein detection and quantitation from mouse tissue extracts or plasma samples using electroluminescence (ECL) technology on the Meso Scale Discovery (MSD) Sector S600 device.

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qPCR

This protocol automates RTqPCR on the Transcriptic platform. 

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RNA Isolation

This protocol automates RNA isolation from bacterial cultures and mouse liver tissue samples using magnetic bead-based technology on the Transcriptic platform.

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