Protocol Applications

Basic PCR

This protocol automates PCR on the Transcriptic platform. 

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CRISPR

This workflow takes an empty CRISPR vector of your choice along with oligos based on your guide sequence and delivers final assemblies - ready for you to transform - 96 at a time.

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ELISA

This protocol automates colorimetric protein detection from liver tissue extracts or plasma samples using enzyme-linked immunosorbent assay (ELISA) technology on the Transcriptic platform.

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Exo-SAP IT

This protocol automates ExoSap-IT treatment for PCR purification on the Transcriptic platform. 

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Genotyping

This protocol automates Genotyping on the Transcriptic platform. 

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Kunkel Mutagenesis

This method uses a phage-derived uracil-containing ssDNA template and mutagenic oligonucleotides to confer targeted mutations to a plasmid of interest. 

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Oligosynthesis

The Oligosynthesis Quick Instruction generates high quality (mass spectroscopy and capillary electrophoresis-verified) oligonucleotides.

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Picogreen Assay

The Picogreen Protocol successfully generated a standard curve using standards of known DNA concentration (0-50 ng/µl).  Plotting of log10[DNA concentration] vs. fluorescence generated a linear trend. 

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Pipetting Tools

This protocol automates High-Throughput Pipetting treatment for PCR purification on the Transcriptic platform. 

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Plasmid Preparation

The Plasmid Preparation Quick Instruction can be used to inoculate and propagate bacterial stocks with successful, high-yield DNA extraction.

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Plate Reading

Assessment of plate reading measurements employing multiple wavelengths between 475 - 535 nm was achieved using two independent experiments, performed in the same measurement plate (96-well, flat-bottom, optically-clear). 

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qPCR

This protocol automates RTqPCR on the Transcriptic platform. 

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Restriction Digestion

This protocol automates Restriction Digestion on the Transcriptic platform. 

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RNA Isolation

This protocol automates RNA isolation from bacterial cultures and mouse liver tissue samples using magnetic bead-based technology on the Transcriptic platform

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Sequencing

This protocol automates Sequencing on the Transcriptic platform. 

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Transform, Spread, Pick

This protocol takes up to 32 DNA aliquots of your choice and Zymo Mix & Go [1] (Zymo Research Corporation) chemically competent cells (Zymo 5α, Zymo 10B, or JM109) to generate individually picked colonies from streaked bacteria. 

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Quikchange Lightning 

This protocol automates the QuikChange Lightning kit, which provides a highly efficient non-PCR method for reliable site-directed mutagenesis.

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