To demonstrate the validity of Transcriptic’s Autoprotocol-implemented Sequencing Quick Instruction.
pBabe-puro plasmid  harboring human K-RAS (isoform B) cDNA was dideoxysequenced by using the Sequencing Quick Instruction. Sequencing primer was generated using the Oligosynthesis Quick Instruction and designed to anneal immediately 3' of K-RAS, forming a complementary interaction with the sense strand.
Sequencing primer design enabled dideoxysequencing with high-quality chromatogram formation from template DNA.
 Morgenstern JP, Land H. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line. Nucleic Acids Res. 1990;12:3587-96
VALIDATION DATE: August 2015